A Sensitive in vitro Method for Prolactin Determination.

Abstract
The mid-part of the inguinal mammary gland of non-primed virgin, metestric albino rats, weighing 270+10 g, is sensitive to 0.001 IU/ml of prolactin when cultivated in enriched synthetic medium M-199 for a period of 2 days. This method may replace the standard crop-gland method for prolactin assay and other routine tests for prolactin, because of its reproducibility, high accuracy and specificity. Its quantitative aspects are being worked out.