Aeromonas hydrophila in acute diarrheal disease: detection of enterotoxin and biotyping of strains

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Abstract
Eleven isolates of Aeromonas spp. from human stool cultures produced enterotoxin activity as determined by assay of culture filtrates in rabbit intestinal loops and rabbit skin and on [mouse] adrenal Y1 cells. Hemolysin(s) and a cytotoxic protein interfered in all 3 assay systems but could be inactivated by heating at 56.degree. C or by specific antihemolysin. Biotyping of each isolate was performed with a conventional test system and with API 50E and APIZYM kit systems (Analytab, Inc.). No single test of the more than 70 biochemical reactions investigated correlated with enterotoxigenicity in the strains of A. hydrophila examined. All strains belonged to ideal phenotypes of A. hydrophila, but each strain possessed its own biochemical profile.