SURVIVAL OF GRANULOCYTIC PROGENITORS IN THE NONADHERENT AND ADHERENT COMPARTMENTS OF HUMAN LONG-TERM MARROW CULTURES

  • 1 January 1984
    • journal article
    • research article
    • Vol. 12  (5) , 327-338
Abstract
The survival of granulocytic and monocytic progenitor cells (CFU-GM) in human liquid marrow cultures was studied. CFU-GM were present in the nonadherent cell population for a mean of 12 wk without recharging. Histochemical analysis of agar gels revealed that most day-7 colonies were of neutrophilic type (CFU-N), whereas the majority of day-14 colonies were of mixed neutrophilic-macrophagic type (CFU-NM) for the first 4 wk of culture and became predominantly of macrophagic type (CFU-M) thereafter. Eosinophilic colonies (CFU-Eo) declined after wk 2 of culture. CFU-GM were present in the adherent layer of these cultures, and the CFU-GM in the nonadherent compartment arise from the adherent layer. The pre-CFU-GM survival was compared in the adherent and nonadherent populations and these progenitors were rapidly depleted from both compartments though their survival at the end of wk 1 was better in the adherent than in the nonadherent layer.