In vitro Effect of Surface Active Agents on Human Serum Lipoprotein and Protein Patterns.

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Abstract
The effects of incubation of serum with surface active agents on serum lipid and protein patterns were studied by electrophoresis and the ultracentrifuge. Direction of migration of lipid in electrophoresis depended upon the charge of these surface active agents. Anionic agents increased migration rate of both alpha and beta lipoproteins toward the anode. Cationic agents either decreased migration rate of lipids toward the anode or caused them to move toward the cathode. Non-ionic agents caused complete loss of mobility of all lipids toward either electrode. These effects could be explained by assuming that surface active agents are bound to lipoproteins and contribute their charge in the migration of these lipoproteins. Effects of surface active agents on protein patterns were less pronounced. Surface active agents either increased or decreased density of serum lipoproteins as studied in the ultracentrifuge suggesting that these agents are bound to lipoproteins. However, prediction of effects of these agents on lipoprotein densities could not be made from knowledge of the charge present on the agent.