In vitro Effect of Soybean Phosphatides on Serum Lipoproteins of Normal and Hyperlipemic Subjects.

Publisher Website
Google Scholar
Abstract
Incubation of 6 normal and 5 hyperlipemic sera with a purified extract of natural soybean phosphatides produced an increase in migration velocity of beta lipoprotein, determined by paper electrophoresis. In 5 of the normal and 1 of the hyperlipemic sera increases in the migration velocity of alpha lipoprotein also were observed. Incubation of 10 normal and 9 hyperlipemic sera with the alcohol-soluble fraction of the phosphatide complex (which consisted of 2/3 lecithin and 1/3 cephalin) produced little or no change in the migration velocities of the serum lipoproteins. Incubation of the sera with the alcohol-insoluble fraction, (which consisted of 2/3 lipositol and 1/3 cephalin) produced an increase in the migration velocities similar to that noted with the whole phosphatide complex. Lipositol may be the compound responsible for the change observed. Determination of free fatty acids after incubation with either fraction failed to disclose any differential liberation of free fatty acids by the 2 fractions. The mechanism by which the alcohol-insoluble lipositol fraction increased the migration velocity of beta lipoprotein remains unexplained.