FACTORS AFFECTING THE PRODUCTION OF BOVINE TYPE-I INTERFERON ON BOVINE EMBRYONIC LUNG-CELLS BY POLYRIBOINOSINIC-POLYRIBOCYTIDYLIC ACID

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  • 1 January 1980
    • journal article
    • research article
    • Vol. 41  (4) , 557-560
Abstract
Variables believed to affect the amount of interferon (IF) produced in bovine embryonic lung cell cultures by poly (rI:rC) were investigated. A concentration of 100 .mu.g of poly (rI:rC)/ml consistently induced substantial amounts of IF. Bovine viral diarrhea virus infection of cultures did not interfere with induction of IF by poly (rI:rC). Neither the age of cells nor the passage level of the cultures seemed to affect the amount of IF produced. Priming of cultures with large concentrations of IF before treatment with poly (rI:rC) was more effective than priming with small concentrations of IF. Priming for 24 h was more effective than was priming for 12 h. The use of 2% bovine fetal serum in the medium subsequent to treatment with poly (rI:rC) markedly enhnaced IF production. Practically all IF was produced within a few hours after treatment with poly (rI:rC).