Size Exclusion Chromatography of Poly(vinylpyrrolidone): I. The Chromatographic Method

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Abstract
The optimum size exclusion chromatographic (SEC) method for poly-(vinylpyrrolidone) (PVP) was found to be based upon a stationary phase of diol derivatized silica of pore sizes 3000, 500, and 75Å and a mobile phase of 50:50 (v/v) MeOH/H2O containing 0.1M-LiNO3. Sample recovery under identical conditions varied for the commercial packings investigated and was found to be inversely related to molecular weight. The latter phenomenon was rationalized on the basis of a limited number of active substrate sites available for binding. Methanol was found to be a more effective mobile phase modifier than either dimethyl formamide or acetonitrile apparently due to its ability to function as a proton donor in hydrogen bonding with PVP. Chromatographic evidence for the existence of semipolyampholyte character in PVP is presented. A procedure for the construction of a column set log-linear in calibration and of extended dynamic range is described and is based upon hydrodynamic volume theory.