The lysing action of enzymes on a sample of mycelium of Penicillium griseofulvum Dierckx

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Abstract
Air-dried mycelium of P. griseofulvum was subjected to autolysis. The suspended matter in the final product contained 16% of the original protein, 64% of the carbohydrate and 50% of the chitin. The soln. contained dispersed protein and carbohydrate, but an alpha-amino acids or reducing sugar. Air-dried mycelium after 2.5-3 yrs. storage was autolyzed. The suspended material contained 39% of the original protein, 71% of the carbohydrate and 70% of the chitin. The soln. contained amino acids, reducing sugar and N-acetyl glucosamine. The protease of the mycelium is stable to storage. Much polysaccharide-splitting and chitinase activity is lost on drying, but some of the residual activity remains after 3 yrs. Such stored mycelium contains a very weak glucose oxidase, but no alpha-amino-acid oxidase, which was presumably very active in the fresh dry mycelium. Little change takes place in the composition of the mycelium on storage except for a slight hydrolysis of protein. 10-15% of the mycelium protein resists digestion with proteases; 10-25% of the carbohydrate and about 10% of the chitin resist digestion with juice from the alimentary tract of snails.
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