Rat IgD myeloma protein: cell‐free translation of the δ mRNA and biochemical analysis of intracellular and membrane δ chain

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Abstract
The analysis of IgD molecules produced by the tumor‐derived IR 731 cell line showed that these molecules are not secreted and are only present at the cell surface as covalent dimers of 48000 dalton δ chains. The original IR 731 tumor secreted complete IgD; thus, the disappearence of secretion in the cell line was correlated with the absence of light chain synthesis. The mRNA coding for the cell line δ chain was isolated and translated. The analysis of the translated δ chain (mol. wt.: 40000) confirmed the absence of a region having the size of a domain as previously shown by partial amino acid sequence. The observation that the absence in δ chain of this region, which comprises most of the CH2 domain, could be a general feature of murine species is discussed.