Liquid scintillation-counting for [14C]steroids

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Abstract
The assay of Cl4-labeled steroids with the Ekeo type N 612 liquid scintillation counter is described. The effects of scintillator volumes and concentration on the efficiency of the method are given. Self-quenching by 25 different steroids has been examined. None of the steroids, at the levels tested, exerted strong quenching effects, although slight quenching was apparent with some, in particular with equilenin and estrone. The effect of impurities in the scintillator on the efficiency of the method were investigated. Some additional variables which affect the reproducibility are discussed.