Synthesis of fluorescent nucleotide analogues: 5'-mono-, di-, and triphosphates of linear-benzoguanosine, linear-benzoinosine, and linear-benzoxanthosine.

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Abstract
The fluorescent nucleotide analogs (the 5''-mono-, di and triphosphates of lin-benzoguanosine, lin-benzoxanthosine and lin-benzoinosine) were prepared for use as dimensional probes of enzyme binding sites. They have quantum yields in aqueous solution of 0.39, 0.55 and 0.04 and fluorescent lifetimes of 6, 9 and .apprxeq. 1.5 ns, respectively. lin-Benzoinosine-5''-monophosphate is a substrate for xanthine oxidase (xanthine:oxygen oxidoreductase, EC 1.2.3.2), providing lin-benzoxanthosine-5''-monophosphate and lin-benzoinosine 5''-diphosphate is a substrate for polynucleotide phosphorylase (polyribonucleotide:orthophosphate nucleotidyltransferase, EC 2.7.7.8), giving poly(lin-benzoinosinic acid). The benzologues of the purine diphosphates are substrates for pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC 2.7.1.40), which is used to prepare the triphosphates.