Antibodies to guanosine

15 October 1984

journal article
Published by Wiley in FEBS Letters

Vol. 176 (1) , 43-48
https://doi.org/10.1016/0014-5793(84)80908-4

Abstract

Bovine serum albumin conjugates of guanosine prepared by the periodate method was used as immunogen to elicit guanosine antibodies in rabbits. The specificities of the antibodies were studied by the inhibition of their binding to [³H]G^ox‐red, [³²P]DNA and [³H]RNA by related non‐radioactive compounds. A population of antibodies is specific to G^ox‐red with an average association constant of around 10⁷M⁻¹ at 4°C. There are a population of antibodies which bind to [³²P]ssDNA and [³H]RNA specifically at guanosine residues. RNA binding antibodies were separated into two populations by affinity chromatography.

Keywords

This publication has 13 references indexed in Scilit:

[3] The experimental induction of antibodies to nucleic acids
Published by Elsevier ,1980
Antibodies Specific for Modified Nucleosides: An Immunochemical Approach for the Isolation and Characterization of Nucleic Acids
Progress in Nucleic Acid Research and Molecular Biology, 1980
The Specificity and Applications of Antibodies to Helical Nucleic Acid
CRC Critical Reviews in Biochemistry, 1975
Specificity of anti-nucleoside antibodies
Biochemical Journal, 1974
A simplified method for cyanogen bromide activation of agarose for affinity chromatography
Analytical Biochemistry, 1974
Immunologic studies on nucleic acids and their components. I. An analysis of the specificity of anti-deoxyadenylate antibodies by a membrane-binding technique
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
Specific binding of ribonucleic acid by antiadenosine antibodies
Biochemistry, 1973
Nucleic Acid Antigens
Published by Elsevier ,1973
ANTIBODIES SPECIFIC FOR RIBONUCLEOSIDES AND RIBONUCLEOTIDES AND THEIR REACTION WITH DNA
Proceedings of the National Academy of Sciences, 1964
A Simple Chromatographic Method for Preparation of Gamma Globulin.
Experimental Biology and Medicine, 1960