Removal of 5′-terminal m7G from eukaryotic mRNAs by potato nucleotide pyrophosphatase and its effect on translation
Open Access
- 1 September 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 4 (9) , 3065-3082
- https://doi.org/10.1093/nar/4.9.3065
Abstract
The procedure for isolation of nucleotide pyrophosphatase (E.C. 3.6.1.9.) from potato has been modified to yield an endonuclease-free preparation purified 2300–fold. The enzyme was used for specific cleavage of pyrophosphate linkages in the 5′-terminal cap (m7GpppN) of several eukaryotic messenger RNAs. Enzymatic removal of 5′-terminal pm7G from reovirus, rabbit globin and Artemia salina mRNAs resulted in an almost complete loss (>80%) of their template activities in a cell-free protein synthesizing system from wheat germ. Incubation with nucleotide pyrophosphatase did not decrease the translation of phage f2 RNA in an Escherichia coli cell-free system.Keywords
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