Protein-Bound Iodine (PBI)—Investigation into Barkers Distillation Method

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Abstract
Mention is made of some investigations into Barker''s distillation method for the determination of protein-bound iodine (PBI) in serum. By adding varying amounts of KI to serum, the PBI was increased by amounts of 10 [mu]g I-/2 ml serum, while smaller amounts were washed away completely; however, the majority of 0.2 [mu]g thyroxin iodine could be recovered. The importance is stressed of digesting only up to the temperature where H2SO4 fumes appear, and of adhering strictly to the distillation period. An account is given of the accuracy of the method used in our laboratory. The standard deviation for repeated determination of the same serum in 6 different cases was [plus or minus] 0.44 [mu]g/100 ml.