The Use of Monoclonal Antibodies to Detect Wheat Soil-borne Mosaic Virus

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Abstract
Stable hybridoma cell lines secreting monoclonal antibodies (MAbs) to wheat soil-borne mosaic virus (WSBMV) were produced by fusing spleen cells of immunized BALB/c mice and mouse myeloma cell line P3-X63-Ag8.653. Hybridoma clones produced antibodies of the IgG2a, IgG2b and IgG3 subclasses. Epitope analysis suggested that all the antibodies tested reacted with the same or closely adjacent antigenic sites. The MAbs reacted with particles of four isolates of WSBMV, but not with particles of 13 other viruses. The serological reactivities of the MAbs were compared with those of rabbit polyclonal antibodies for the detection of WSBMV in leaf tissue by ELISA. ELISA using both polyclonal antibodies and MAbs was superior to assays using either source of antibody alone. The MAbs of the IgG2b subclass worked well in a Protein A sandwich ELISA, and a dot immunobinding assay using only MAbs was also satisfactory for the detection of WSBMV.