A Spectrophotometric Study of the Copigmentation of Malvin with Caffeic and Ferulic Acids

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Abstract
The process of copigmentation of the anthocyanin molecule malvidin 3, 5-diglucoside with two organic monocarboxylic phenolic acids, caffeic and ferulic acids, was studied via their absorption electronic spectra. The dependence of the copigmentation process on the pH of the medium, molecular concentration, and temperature was established. The process of copigmentation was observed at two pH values: 2.50 and 3.65. The stoichiometric ratio was 1:1 at both pH values. The copigmentation was characterized by approximately equal values of the equilibrium constant, K, within each of the pH values. The temperature was found to be a significant factor that determines the thermodynamic conditions of the copigmentation process, because the process is spontaneous (DeltaG degrees < 0), and results in entropy loss (DeltaS degrees < 0) at both pH values.