Sodium deoxycholate-polyacrylamide gel electrophoresis of lipopolysaccharides at low temperature.

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Abstract
Sodium deoxycholate (DOC)-polyacrylamide gel electrophoresis (PAGE) has previously been used to examine in detail the chemical heterogeneity of lipopolysaccharide (LPS). It is now shown that DOC-PAGE carried out at 4 C can give reproducible banding patterns even when a large amount of LPS (20 mg) is applied to the slab gel (4 .times. 150 .times. 300 mm). By DOC-PAGE, we dound that LPS obtained from E. coli UKT-B showed 14 silver-straining bands on gels but lacked the bands corresponding to a series of high-molecular-weight LPS usually observed in the S form LPS. These findings suggest that the DOC-PAGE at 4.degree. C should be useful in fractionating LPS intactly to study LPS preparations with unknown structures.