Folic Acid in Axenic Cultures of Neoaplectana

Abstract
Neoaplectana glaseri, a nematode parasite of insects, needs folic acid for reproduc-tioa This has been shown in axenic cultures by omitting the vitamin from chemically defined medium or including antimetabolites such as aminopterin and amethopterin in a meat infusion broth. Bacterial assays for folic and folinic acids show quantitative changes in various media inoculated with N. glaseri and two other parasitic species which do not thrive as well in liquids, N. dutkii and N. carpocapsae.