Cerebral diphosphoinositide breakdown: activation, complexity and distribution in animal (mainly nervous) tissues

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Abstract
In a suitable medium the reaction between cerebral-tissue suspensions and the "inositol phosphatide" fraction of brain cephalin results in the concurrent release of inositol monophosphate and smaller amounts of inorganic phosphate. Inositol monophosphate release is activated by Ca salts, whilst inorganic phosphate release is slightly inhibited by Ca, Mg and Ba salts. The purified K salt of diphosphoinositide is attacked by cerebral-tissue suspensions at a rate equivalent to 300 u moles/g fresh weight of tissue/hour, which is at least 6 times as rapid as the reaction with "inositol phosphatide". The enzyme system is present in suspensions prepared from the brains of the guinea pig, rat, rabbit, monkey, sheep, and infant and adult humans. No great variations in activity have been found in gross anatomical regions of the central nervous system. Suspensions of liver, spleen and kidney also attack "inositol phosphatide". The reaction was not influenced significantly by several enzyme inhibitors, drugs and compounds structurally related to inositol.