Small Maf proteins interact with the human transcription factor TCF11/Nrf1/LCR-F1
- 1 November 1996
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 24 (21) , 4289-4297
- https://doi.org/10.1093/nar/24.21.4289
Abstract
The human TCF11 gene encodes a ubiquitously expressed bZIP transcription factor of the cap n' collar (CNC) domain family. It has a high sequence similarity to the erythroid-specific bZIP factor p45 NF-E2 in the CNC domain, which is involved in DNA binding. LCR-F1, a TCF11 isoform, is a more potent transcriptional activator than p45 NF-E2 in erythroid cells. We show here that the TCF11 protein interacts to form heterodimers with small Maf proteins, previously shown to dimerize with p45 NF-E2, ECH and Fos. Such heterodimerization significantly alters the DNA binding characteristics of TCF11. While TCF11 alone binds in vitro to the tandem NF-E2 site derived from 5' DNase hypersensitive site 2 in the beta-globin locus control region and to the single NF-E2 site in the porphobilinogen deaminase gene promoter, stronger binding is detected in the presence of small Maf proteins. Using antibodies, TCF11 isoforms bound to the single NF-E2 site were detected in K562 erythroid cell nuclear extracts. These findings place TCF11 as a good candidate for the proposed widely expressed factor(s) known to interact with small Maf proteins and bind NF-E2 sites in a sequence-specific manner resembling NF-E2.Keywords
This publication has 28 references indexed in Scilit:
- Complexity of the erythroid transcription factor NF-E2 as revealed by gene targeting of the mouse p18 NF-E2 locus.Proceedings of the National Academy of Sciences, 1996
- Purification of the human NF-E2 complex: cDNA cloning of the hematopoietic cell-specific subunit and evidence for an associated partner.Molecular and Cellular Biology, 1993
- Erythroid transcription factor NF-E2 is a haematopoietic-specific basic–leucine zipper proteinNature, 1993
- Segmentally restricted, cephalic expression of a leucine zipper gene during Drosophila embryogenesisMechanisms of Development, 1991
- Tandem AP-1-binding sites within the human beta-globin dominant control region function as an inducible enhancer in erythroid cells.Genes & Development, 1990
- v-maf, a viral oncogene that encodes a "leucine zipper" motif.Proceedings of the National Academy of Sciences, 1989
- Cis- and trans-acting elements involved in the regulation of the erythroid promoter of the human porphobilinogen deaminase gene.Proceedings of the National Academy of Sciences, 1989
- Rapid detection of octamer binding proteins with ‘mini extracts’, prepared from a small number of cellsNucleic Acids Research, 1989
- Two tissue-specific factors bind the erythroid promoter of the human porphobilinogen deaminase geneNucleic Acids Research, 1989
- A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samplesAnalytical Biochemistry, 1978