THE OXIDATION OF COMPOUNDS RELATED TO THE TRICARBOXYLIC ACID CYCLE BY WHOLE CELLS AND ENZYME PREPARATIONS OF MYCOBACTERIUM TUBERCULOSIS VAR. HOMINIS

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Abstract
Substrates related to the tricarboxylic acid cycle stimulate respiration of enzyme preparations of the highly virulent strain H37Rv of M. tuberculosis var. hominis prepared by drying cells with acetone. However, of these substrates only lactic, pyruvic, oxalosuccinic, a ketoglutaric, acetic acids and dl-alanine stimulate O2 uptake of the whole mycobacterial cells. Radioactive acetate is oxidized by way of this cycle, since on isolation by anion exchange chromatography, glutamic, aspartic, lactic, pyruvic, citric, isocitric, cis-aconitic, a ketoglutaric, malic, fumaric, and possibly succinic contain radioactive C. By employing paper partition chromatography, radioactive citrate was found.