Biosynthesis of vitamin B12: concerning the origin of the methine protons of the corrin nucleus.
- 1 October 1987
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 84 (19) , 6616-6618
- https://doi.org/10.1073/pnas.84.19.6616
Abstract
13C NMR spectroscopy has been used to locate six deuterium atoms incorporated biosynthetically on the periphery of the corrin nucleus of vitamin B12 (cyanocobalamin) derived from cells of Propionibacterium shermanii grown in a medium containing 50% 2H2O and 13C-enriched .delta.-aminolevulinic acid. The implications of these results for the mechanism of vitamin B12 biosynthesis are discussed, and it is concluded that the same oxidation level of the intermediate is maintained throughout the biosynthetic pathway, from .delta.-aminolevulinic acid to corrin.This publication has 7 references indexed in Scilit:
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