Ovine lactoferrin: isolation from colostrum and characterization
- 1 May 1991
- journal article
- research article
- Published by Cambridge University Press (CUP) in Journal of Dairy Research
- Vol. 58 (2) , 211-218
- https://doi.org/10.1017/s0022029900029757
Abstract
Summary Highly purified lactoferrin was isolated from ovine colostrum by sequential purification on CM-Sephadex C-50 and Blue-Sepharose, with overall yield of 55%. The ovine lactoferrin was characterized by SDS-PAGE, its amino acid composition and N-terminal sequence to residue 30. Homology with bovine and human lactoferrins was greater than 80 and 50% respectively. Antibodies to ovine lactoferrin were raised in rabbits and used to develop an enzyme-linked immuno-sorbent assay (ELISA). The antiserum was not cross reactive with other colostrum proteins.Keywords
This publication has 19 references indexed in Scilit:
- One-Step Isolation of Lactoferrin Using Immobilized Monoclonal AntibodiesJournal of Dairy Science, 1987
- Isolation and characterisation of lactoferrin separated from human whey by adsorption chromatography using Cibacron Blue F3G-A linked affinity adsorbentClinica Chimica Acta; International Journal of Clinical Chemistry, 1986
- Human lactotransferrin: amino acid sequence and structural comparisons with other transferrinsEuropean Journal of Biochemistry, 1984
- Isolation of Lactoferrin and Its Concentration in Sows' Colostrum and Milk during a 21-Day Lactation2Journal of Animal Science, 1984
- The fractionation and characterization of bovine tear proteins, especially lactoferrinMolecular and Cellular Biochemistry, 1982
- Biochemical Properties of Bovine GranulocytesExperimental Biology and Medicine, 1978
- Iron-binding Proteins in Milk and Resistance to Escherichia coli Infection in InfantsBMJ, 1972
- ASSOCIATION OF LACTOFERRIN WITH SPECIFIC GRANULES IN RABBIT HETEROPHIL LEUKOCYTESThe Journal of Experimental Medicine, 1970
- Preparation of some iron-binding proteins and α-lactalbumin from bovine milkBiochimica et Biophysica Acta (BBA) - General Subjects, 1965
- The Isolation of a Red Protein from Milk2Journal of the American Chemical Society, 1960