Quantitative Analysis of Vitamin D3 in a Feed Using Normal Phase High Pressure Liquid Chromatography
- 1 September 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 17 (9) , 510-513
- https://doi.org/10.1093/chromsci/17.9.510
Abstract
A procedure is described for the separation and quantification of Vitamin D3 from different feeds and premixes. The study was conducted, first using a liquid partition step as a preliminary clean-up after extraction, then chromatography on activated Silica gel 60 before final analysis on a high pressure liquid chromatograph (HPLC) using a LiChrosorb NH2 (10μ) column and a variable wavelength UV detector set at 264 nm. Total analysis on the HPLC was achieved in fifteen minutes. The detector response curve for an authentic D3 standard was linear using peak areas with a minimum detectable amount being 5 ng. The overall percent recovery of D3 in feeds was 94.4 ± 2.4%. The minimum detectable amount of D3 in animal feeds was found to be in the region of 2,000 I.U./kg.This publication has 4 references indexed in Scilit:
- High-performance liquid chromatography determination of potential content of vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol) in resins, oils, dry concentrates and multivitamin formulationsJournal of Chromatography A, 1978
- Method for the extraction and cleanup of animal feed for the determination of liposoluble vitamins D, A, and E by high-pressure liquid chromatographyJournal of Agricultural and Food Chemistry, 1978
- Analysis of Fat-Soluble Vitamins. XVI. Antirachitic Activity of 5,6-trans-Vitamin D3 Alone and in the Presence of 5,6-cis-Vitamin D3 Resin, Using Chick BioassaysJournal of AOAC INTERNATIONAL, 1977
- High-Pressure Liquid Chromatographic Determination of Vitamin D3 in Resins, Oils, Dry Concentrates, and Dry Concentrates Containing Vitamin AJournal of AOAC INTERNATIONAL, 1976