The effect of mode of agitation and type of plastic bag on storage characteristics and in vivo kinetics of platelet concentrates

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Abstract
We studied the characteristics of platelet concentrates stored for 5 days at 22°C. Platelets were prepared in three plastic bags (PL 732, PL 1240, and CLX) and stored on one of four platelet agitators, 1‐ or 6‐rpm elliptical and 2‐ or 6‐rpm circular rotators. A total of 76 studies were divided among 12 groups, each group being composed of a different storage bagrotator combination. In vivo recovery and survival were calculated using Indium‐111 oxine‐labeled platelets injected into autologous volunteers. Platelet recovery was assessed at 2 hours postinjection or as the y‐intercept of the multiple‐hit model survival curve. Survival was calculated using linear, exponential, and multiple‐hit computer models. Linear T 1/2 also was calculated as an index of platelet survival.At 5 days, the pH of all concentrates was above pH 7.0 and platelet counts were above 5.5 × 1010 per bag except for the PL 732 with the 6‐rpm elliptical rotator, which was 4.6 × 1010 per bag. This combination also showed a significantly higher poststorage lactic dehydrogenase (LDH) discharge compared to the mean of the other 11 groups (23.6 ± 5.4% vs. 10.4 ± 3.0%, p < 0.05); however, the β‐thromboglobulin (β‐TG) release was not statistically different. The lowest in vivo recovery and survival T 1/2 were found for the PL 732 with the 6‐rpm elliptical rotator: 2‐hour recovery of 28.0 ± 3.2 percent vs. 40.0 ± 10.6 percent for the other 11 groups (p < 0.05) and survival T 1/2 of 2.1 ± 1.4 days vs. 2.9 ± 0.6 days (p<0.05). Survivals using the three computer models, however, were similar for all 12 groups and were 7.2 ± 1.3 days (linear), 3.3 ± 0.9 days (exponential), 5.7 ± 2.0 days (multiple hit) (n = 76). The percent in vitro release of β‐TG or discharge of LDH during storage did not predict in vivo recovery. We conclude that storage of platelet concentrates for 5 days in PL 732 containers on a 6‐rpm elliptical rotator is the least desirable method of storage; all other combinations gave acceptable and similar results.
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