HUMAN-PLATELETS LABELED WITH IN-111 8-HYDROXYQUINOLINE - KINETICS, DISTRIBUTION, AND ESTIMATES OF RADIATION-DOSE

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  • 1 January 1982
    • journal article
    • research article
    • Vol. 23  (2) , 149-156
Abstract
Platelets from 9 normal male subjects were labeled with 111In 8-hydroxyquinoline (111In oxine) in the presence of plasma in either closed blood transfer packs or in open test tubes. The mean labeling efficiencies in these 2 systems were 27 and 53%, respectively. Mean survival time of 111In-labeled autologous platelets was 8.76 days, with a SD of 1.05 according to the maximum-likelihood estimate of the .gamma.-function model. The initital recovery of 111In platelets in the circulation was 57% with a SD of 11%. The distribution of 111In platelets in liver and spleen was quantitated by anterior, posterior and transmission .gamma.-camera imaging. During the first 30 min, 38% of the injected dose accumulated in the spleen, 13% in the liver. No significant increase in 11In radioactivity was observed in either of the 2 organs over a 3-9 day period. The bone marrow was an additional site of 11In accumulation. The spleen was the critical organ with respect to radiation dose. The splenic dose was 34 rad/mCi 111In platelets, that of the liver 2.1 rad/mCi. With the injection of 100-150 .mu.Ci of 111In-labeled platelets in normal subjects, giving a splenic radiation of 5 rad, a complete 10-day survival study can be performed and uptake of 111In in different organs can be measured quantitatively for at least 3-4 days.