EFFECT OF BOVINE GROWTH-HORMONE ON DEVELOPMENT OF GOAT MAMMARY TISSUE IN ORGAN-CULTURE

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  • 1 January 1982
    • journal article
    • research article
    • Vol. 16  (1) , 19-31
Abstract
The effect of bovine growth hormone (bGH) on DNA, protein and casein synthesis in goat mammary explants was studied. GH was unable to stimulate DNA synthesis or potentiate insulin-stimulated DNA synthesis either in high or low O2 concentrations. In the presence of insulin and cortisol bGH had no effect on the synthesis of explant cytosol proteins. Two preparations of bGH were tested for their effect on casein synthesis. The preparation NIH[National Institutes of Health]-GH-B17 at concentration 50 .mu.g ml-1 increased casein synthesis similarly as .apprx. 0.5 .mu.g ml-1 of prolactin. The preparation, at concentration 50 .mu.g ml-1 had effect lower than 0.5 .mu.g ml-1 of prolactin. The lactogenic activity of NIH-GH-B17 was decreased by treatment with specific antisera to bovine prolactin. When submaximum concentration of prolactin (0.05 .mu.g ml-1) was added, bGH at concentration as low as 0.5 .mu.g ml-1 had a synergistic effect on prolactin stimulated casein synthesis.