FINE MAPPING ANALYSIS OF THE AMYLASE GENE IN BACILLUS SUBTILIS BY TRANSFORMATION

Abstract
In the genetic analysis of the amylase gene (amy) of B. subtilis, phe (phenylalanine) and aro (aromatic amino-acids) were found to be linked to amy. These 2 mutations were closely linked to each other. Sixteen isolated amylase negative mutations were mapped in a single locus by the transformation technique.