Bradykinin‐induced transient accumulation of inositol trisphosphate in neuron‐like cell line NG 108‐15 cells

Abstract
Studies were undertaken to further elucidate the mechanism(s) by which bradykinin‐dependent phosphoinositide metabolism takes place in neuroblastoma × glioma hybrid NG108‐15 cells [(1984) J. Biol. Chem. 259, 10201–10207] using [3H]inositol‐labelled cells. Bradykinin produced net increases in the level of [3H]inositol phosphates, especially of [3H]inositol trisphosphate which is formed transiently and most rapidly. The results indicate that bradykinin activates a phosphodiesterase to break down phosphatidylinositol 4,5‐bisphosphate, generating two recently recognized intracellular messengers, 1,2‐diacylglycerol and inositol trisphosphate.