Nucleotide exchange reactions catalysed by ribonuclease and spleen phosphodiesterase. 2. Synthesis of polynucleotides

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Abstract
Ribonuclease catalyzed the synthesis of polynucleotides from cyclic cytidylic acid (cytidine-23[image]phosphate). Evidence for synthesis of the dinucleotide, cyclic dinucleotide and cyclic trinucleotide was presented. With cytidine also present as an acceptor molecule, 2 other reaction products were isolated, namely the dinucleoside phosphate and the tri-nucleoside diphosphate. Under certain conditions the amount of synthesis well exceeded the extent of hydrolysis of the cyclic mono-nucleotides. Uridine derivatives were also active in these reactions. In the transfer reactions catalyzed by ribonuclease, adenosine and adenosine-2[image]:3[image] phosphate could be used also as acceptor molecules. In general, the nucleoside -3[image] phosphates were very poor as acceptors. A phosphodiesterase preparation purified from spleen catalyzed exchange reactions involving ribopolynucleotides. This enzyme preparation, however, was inactive with cyclic nucleotides. The possible significance of these reactions in ribonucleic acid interconversions was discussed.