Identification of Luciferyl Adenylate and Luciferyl Coenzyme A Synthesized by Firefly Luciferase
- 19 December 2003
- journal article
- research article
- Published by Wiley in ChemBioChem
- Vol. 5 (1) , 110-115
- https://doi.org/10.1002/cbic.200300735
Abstract
The firefly luciferase reaction intermediate luciferyl adenylate was detected by RP-HPLC analysis when the luciferase reaction was performed under a nitrogen atmosphere. Although this compound is always specified as an intermediate in the light-production reaction, this is the first report of its identification by HPLC in a luciferase assay medium. Under a low-oxygen atmosphere, luciferase can catalyze the synthesis of luciferyl coenzyme A from luciferin, ATP, and coenzyme A, but in air dehydroluciferyl coenzyme A was produced. The luciferase-catalyzed synthesis of these coenzyme A derivatives may be a consequence of the postulated recent evolutionary origin of firefly luciferases from an ancestral acyl-coenzyme A synthetase.Keywords
This publication has 26 references indexed in Scilit:
- pH opposite effects on synthesis of dinucleoside polyphosphates and on oxidation reactions catalyzed by firefly luciferaseFEBS Letters, 2003
- Dehydroluciferyl‐AMP is the main intermediate in the luciferin dependent synthesis of Ap4A catalyzed by firefly luciferaseFEBS Letters, 1998
- Acyl-Adenylate Motif of the Acyl-Adenylate/Thioester-Forming Enzyme Superfamily: A Site-Directed Mutagenesis Study with the Pseudomonas sp. Strain CBS3 4-Chlorobenzoate:Coenzyme A LigaseBiochemistry, 1997
- Synthesis of Dehydroluciferin by Firefly Luciferase: Effect of Dehydroluciferin, Coenzyme A and Nucleoside Triphosphates on the Luminescent ReactionBiochemical and Biophysical Research Communications, 1997
- Time course of luciferyl adenylate synthesis in the firefly luciferase reactionFEBS Letters, 1996
- Does the sulfhydryl or the adenine moiety of CoA enhance firefly luciferase activity?Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1995
- Firefly luciferase synthesizes P1,P4‐bis(5'‐adenosyl)tetraphosphate (Ap4A) and other dinucleoside polyphosphatesFEBS Letters, 1990
- Improved synthesis of firefly D-luciferyl-D-adenylate - A key intermediate of firefly bioluminescence.Agricultural and Biological Chemistry, 1988
- The function of coenzyme A in luminescenceBiochimica et Biophysica Acta, 1958
- The effect of oxygen upon the immobilization reaction in firefly luminescenceJournal of Cellular and Comparative Physiology, 1953