In vitro plantlet regeneration through asexual embryogenesis in cotyledonary segments of Corylus avellana L.
- 1 October 1983
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 2 (5) , 226-228
- https://doi.org/10.1007/bf00269146
Abstract
Cotyledonary nodes of corylus avellana L. excised from young seedlings cultured in K(h) medium for 5 weeks were used to induce embryoid formation. Embryogenesis was achieved in 60% of the explants over two 20-day culture steps in the presence of IBA (5 μM) plus BAP (0.5 μM) and BAP (5 μM) plus IBA (0.5 μM) respectively. Subsequent proliferation was successfully maintained during 5 subcultures on K(h) basal medium and in an unlimited number of subcultures in the presence of BAP (0.5 μM). Plant regeneration (50–55%) was easily achieved by planting into a basal K(h) medium after 10–20 days.Keywords
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