The Urease Activity of Fluorescent Pseudomonads

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Abstract
SUMMARY: Media frequently used for the detection of urease activity were found to be unsuitable for fluorescent pseudomonads since the presence of free ammonia was found to suppress the formation of urease in growing cultures. Incubation of cultures for 40 hr at 25° in a low concentration Casitone + yeast-extract + glucose medium, followed by the additionof urea to 0.2% (w/v), resulted in the development of an alkaline shift in the medium after further incubation for a few more hours at 37°. This method gave good results in the detection of urease activity.