SPECIFIC BINDING OF LYMPHOCYTES-T TO MACROPHAGES .5. ROLE OF IA ANTIGENS ON M-PHI IN THE BINDING

  • 1 January 1979
    • journal article
    • research article
    • Vol. 122  (4) , 1598-1600
Abstract
The effect of anti-Ia [immune response-associated antigen (Ag)] alloantiserum on the capacity of selected [guinea-pig] peritoneal exudate lymphocytes (selected PEL) to bind to Ag-pulsed F1 (responder .times. nonresponder) macrophages (MO) was investigated. Use of selected PEL for Ag under Ir gene control showed that anti-Ia serum to the responder haplotype blocked adherence of selected PEL to Ag-pulsed macrophages, whereas anti-Ia serum to the nonresponder haplotype did not. The target cell of the anti-Ia alloantiserum appeared to be the macrophage because anti-13 Ia in contrast to anti-2 Ia did not inhibit binding of F1 (2 .times. 13) DNP-GL [dinitrophenylated copolymer of glutamic acid and lysine] selected PEL to DNP-GL pulsed strain-2 M.vphi. (responder strain). An antibody to the native protein Ag employed is unable to block specific binding. T [thymus-derived] cells may recognize fragments of exogenous antigen in association with Ia molecules.