Template functions in the enzymic formation of polyribonucleotides. 3. Apurinic acid as template.
- 1 October 1966
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 56 (4) , 1241-1246
- https://doi.org/10.1073/pnas.56.4.1241
Abstract
When apurinic acid, instead of intact DNA, was used as template in the RNA polymerase system in the presence of all four rlbonucleoslde trlphosphate precursors, a polyrlbonucleotide was synthesized containing a high proportion of adenine and smaller proportions of guanine and, unexpectedly, uracil. Cytosine was not incorporated. The rate of product formation depended on the concentration of apurinic acid, showed an optimum temperature of incubation at 30[degree], and was stimulated by the addition of apyrimidinic acid, which in itself had no template activity. Observations on the use of gel-filtration for the study of this product are mentioned briefly. These studies are being continued.This publication has 16 references indexed in Scilit:
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