Evaluation of an AutoAnalyzer method for quantitating anti-A and anti-B haemagglutinins in factor VIII preparations.

Abstract
Attention has been drawn to instances of hemolysis after intensive therapy of group A, B and AB hemophiliacs with factor VIII preparations containing variable quantities of anti-A and anti-B. The continuous flow principle employed in the Technicon autoanalyzer was adapted for the assay of [human] anti-A and anti-B. The method has an acceptable reproducibility degree and was used principally for the quantitation of anti-A and anti-B in factor VIII concentrates of intermediate activity. It is a method that can be applied to the assay of these antibodies in serum samples.