Microbial Transformation of Antibiotics: Phosphorylation of Clindamycin byStreptomyces coelicolorMüller

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Abstract
Addition of clindamycin to whole-cell cultures ofStreptomyces coelicolorMüller resulted in the loss of in vitro activity against organisms sensitive to clindamycin. Incubation of such culture filtrates with alkaline phosphatase generated a biologically active material identified as clindamycin. Fermentation broths containing inactivated clindamycin yielded clindamycin 3-phosphate, the structure of which was established by physical-chemical and enzymatic studies. Clindamycin was phosphorylated by lysates and partially purified enzyme preparations fromS. coelicolorMüller. These reactions require a ribonucleoside triphosphate and Mg2+. The product of the cell-free reactions was identified as clindamycin 3-phosphate.