LEUKEMIC DISTRIBUTION OF A HUMAN ACUTE LYMPHOCYTIC-LEUKEMIA CELL LINE (ICHIKAWA STRAIN) IN NUDE-MICE CONDITIONED WITH WHOLE-BODY IRRADIATION

  • 1 January 1978
    • journal article
    • research article
    • Vol. 38  (10) , 3494-3498
Abstract
Induction of leukemia in nude mice (BALB/c nu/nu) was attempted by inoculation with a human acute lymphocytic leukemia cell line (Ichikawa strain, maintained in an ascitic form). Inoculation of the cells i.v. in normal nude mice failed to produce leukemia. Conditioning with whole-body irradiation (500 rads) resulted in induction of leukemia after i.v. inoculation, especially when such inoculation was performed 3 days after irradiation. The correlation of survival to inoculum size (105-108) was inversely exponential. Leukemic infiltration was noted in the spleen, lymph nodes, bone marrow, meninges, liver, kidneys, etc., as seen in human leukemia. These cells retained their original cytological characteristics, ultrastructural features and surface markers and revealed high terminal deoxynucleotidyltransferase activity as T[thymus]-derived cells. Chromosome analysis revealed aneuploidy in a hypotetraploid range with a mode of 88 chromosomes.