Induction of non-repairable DNA strand breaks by N-ion beams.

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Abstract
DNA strand breaks were induced by N-ion beam (LET∞ = 530 keV/μm) in HMV-I cells, derived from a human malignant melanoma, and were studied using the alkaline elution method. The DNA strand breaks which were detected included single strand breaks, double strand breaks, and alkaline labile sites. The DNA strand breaks increased linearly with 60Co γ-ray doses. Most of these breaks were rejoined during post-irradiation incubation at 37°C; the remainder were considered non-repairable. Non-repairable DNA strand breaks increased quadratically with increases in 60Co γ-ray doses. However, DNA strand breaks increased nearly linearly with increases in doses of N-ions, even after post-irradiation incubation. These correlations of doses with non-repairable DNA strand breaks were similar to those of doses versus cell survival.