A CIRCULAR-DICHROISM STUDY OF THE ENTEROTOXIN FROM CLOSTRIDIUM PERFRINGENS TYPE A
- 1 June 1985
- journal article
- Published by Hindawi Limited in Journal of Food Biochemistry
- Vol. 9 (2) , 137-146
- https://doi.org/10.1111/j.1745-4514.1985.tb00344.x
Abstract
Far-UV circular-dichroism spectra of the enterotoxin from Clostridium perfringens type A were recorded under different conditions, and conformational analysis was performed. The native enterotoxin (pH 6.8) was shown to contain about 80% pleated sheet, 20% random coil and no helix structure. By adding small amounts of the detergent sodium dodecyl sulfate (0–261 mol SDS/mol enterotoxin), the β-sheet structure could gradually be changed to a more α-helical structure. Treatment with 8 M urea resulted in limited alterations of the secondary structure of the enterotoxin, while heat treatment (60°C, 30 min), treatment with 6.5 M guanidine hydrochloride or 0.01 M NaOH led to a dramatic increase in the amount of random coil.Keywords
This publication has 12 references indexed in Scilit:
- The amino acid sequence of the enterotoxin from Clostridium perfringens type AFEBS Letters, 1985
- Identification of a 50,000 Mr protein from rabbit brush border membranes that binds Clostridium perfringens enterotoxinBiochemical and Biophysical Research Communications, 1983
- Inhibition of protein synthesis by a tryptic polypeptide of Clostridium perfringens type a enterotoxinBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1982
- Effects of temperature, pH and detergents on the molecular conformation of the enterotoxin of Clostridium perfringensBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1982
- Conformational properties of central nervous system myelin basic protein, β‐endorphin, and β‐lipotropin in water and in the presence of anionic lipidsBiopolymers, 1981
- Trypsin activation of enterotoxin from Clostridium perfringens type A fragmentation and some physicochemical propertiesBiochimica et Biophysica Acta (BBA) - Protein Structure, 1981
- Information content in the circular dichroism of proteinsBiochemistry, 1981
- Effect of long-chain alkyl sulfate binding on circular dichroism and conformation of soybean trypsin inhibitorBiochemistry, 1981
- Anomalous aggregation of Clostridium perfringens enterotoxin under dissociating conditionsCanadian Journal of Microbiology, 1976
- Computed circular dichroism spectra for the evaluation of protein conformationBiochemistry, 1969