DNA-mediated gene transfer of beta-aspartylhydroxamate resistance into Chinese hamster ovary cells.
- 1 September 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (9) , 5724-5728
- https://doi.org/10.1073/pnas.78.9.5724
Abstract
Cell lines that have high levels of resistance to .beta.-aspartylhydroxamate and elevated levels of asparagine synthetase activity were selected in 2 steps from Chinese hamster ovary cells. Resistance to .beta.-aspartylhydroxamate was transferred into sensitive cells by using total genomic DNA derived from the dominant 2-step mutants. The surviving colonies were characterized as transferants on the basis of transfer frequency, degree of resistance to .beta.-aspartylhydroxamate, increased level of asparagine synthetase activity, expression of the donor form of asparagine synthetase, codominance in hybrids and instability of the phenotype in the absence of selection.This publication has 23 references indexed in Scilit:
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