THE INSULIN ACTIVITY OF BOVINE AND OVINE BLOOD PLASMA
- 1 September 1962
- journal article
- research article
- Published by Bioscientifica in Journal of Endocrinology
- Vol. 25 (1) , 35-42
- https://doi.org/10.1677/joe.0.0250035
Abstract
SUMMARY: A standardized method for the assay of small amounts of insulin (10–1000 μu./ml.) by measuring the effect on the glucose uptake of rat diaphragm in vitro is described. The most sensitive part of the concentration/response curve is within the range 10–150 μu./ml., and over this range there is a straight-line relationship between insulin concentration and insulin effect. The importance of having a protein such as gelatin present in dilute solutions of insulin is discussed.Keywords
This publication has 6 references indexed in Scilit:
- THE INSULIN ACTIVITY OF BOVINE AND OVINE BLOOD PLASMAJournal of Endocrinology, 1962
- PREVENTION OF INSULIN-I131ADSORPTION TO GLASSEndocrinology, 1961
- Adsorption of Insulin to Glass.Experimental Biology and Medicine, 1959
- THE ASSAY OF INSULIN IN VITRO BY MEANS OF THE GLUCOSE UPTAKE OF THE ISOLATED RAT DIAPHRAGMJournal of Endocrinology, 1956
- ESTIMATION OF PLASMA-INSULIN BY THE RAT DIAPHRAGM METHODThe Lancet, 1954
- EVIDENCE FOR THE PRESENCE OF INSULIN IN BLOOD SERUM. A METHOD FOR AN APPROXIMATE DETERMINATION OF THE INSULIN CONTENT OF BLOOD 1Journal of Clinical Investigation, 1952