Modified oligonucleotides as alternatives to the synthesis of mixed probes for the screening of cDNA libraries.
Open Access
- 1 November 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (22) , 7510-7514
- https://doi.org/10.1073/pnas.82.22.7510
Abstract
Two simple alternatives to the synthesis of mixed oligodeoxyribonucleotide probes are described: mixed deblocking of triazolodeoxynucleosides for the A/G and C/T degeneracies or incorporation of 2-amino-2''-deoxyadenosines into a determined sequence for a higher stability of the hybridization duplexes. The synthetic oligodeoxyribonucleotides obtained were successfully tested in comparison with a classical mixed probe for their capacity to identify specific cDNA clones of human antithrombin III. The results are discussed with respect to the utilization of these synthetic oligonucleotides as hybridization probes for the isolation of cloned cDNA sequences.Keywords
This publication has 20 references indexed in Scilit:
- Sequence requirements for nuclear location of simian virus 40 large-T antigenNature, 1984
- The B–Z transition in two synthetic oligonucleotides: d(C-2-amino-ACGTG) and d(m5CGCAm5CGTGCG) studied by IR, NMR and CD spectroscopiesNucleic Acids Research, 1984
- Human apoHpoprotetas AI, AII, CII and CIII. cDNA sequences and mRNA abundanceNucleic Acids Research, 1984
- Isolation of a genomic clone for bovine pancreatic trypsin inhibitor by using a unique-sequence synthetic DNA probe.Proceedings of the National Academy of Sciences, 1983
- Quick-Blot: Selective mRNA or DNA Immobilization from Whole CellsDNA, 1983
- Use of a cDNA clone for the fourth component of human complement (C4) for analysis of a genetic deficiency of C4 in guinea pig.Proceedings of the National Academy of Sciences, 1983
- The influence of the purine 2-amino group on DNA conformation and stability. Synthesis and conformational analysis of d[T(2-aminoA)]3Nucleic Acids Research, 1982
- The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit β-globin DNANucleic Acids Research, 1981
- Synthesis of 4-triazolopyrimidinone nucleotide and its application in synthesis of 5-methylcytosine-containing oligodeoxyribonucleotidesNucleic Acids Research, 1981
- Hybridization of synthetic oligodeoxyribonucleotides to ΦX174 DNA: the effect of single base pair mismatchNucleic Acids Research, 1979