Molecular cloning and functional expression of the human glycine transporter GlyT2 and chromosomal localisation of the gene in the human genome1
Open Access
- 20 November 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 439 (3) , 334-340
- https://doi.org/10.1016/s0014-5793(98)01390-8
Abstract
Neurotransmitter transport systems are major targets for therapeutic alterations in synaptic function. We have cloned and sequenced a cDNA encoding the human type 2 glycine transporter GlyT2 from human brain and spinal cord. An open reading frame of 2391 nucleotides encodes a 797 amino acid protein that transports glycine in a Na+/Cl−‐dependent manner. When stably expressed in CHO cells, human GlyT2 displays a dose‐dependent uptake of glycine with an apparent K m of 108 μM. This uptake is not affected by sarcosine at concentrations up to 1 mM. Radiation hybrid analysis mapped the GlyT2 gene to D11S1308 (LOD=8.988) on human chromosome 11p15.1–15.2.Keywords
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