Propagation in Tissue Cultures of Cytopathogenic Agents from Patients with Measles.

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Abstract
Eight agents exhibiting the properties of viruses were isolated in cultures of human or simian renal cells from blood or throat washings of 5 cases of typical measles. Multiplication of the agents in vitro is accompanied by characteristic changes in the cells. Primarily these consist in formation of syncytial giant cells wherein the chromatin assumes a marginal position and is replaced centrally by an acidophilic substance of unknown nature. The cytopatho-genic effect of at least 1 of the agents is inhibited by convalescent phase measles sera from other patients with measles. Antigen appears during cultivation in vitro of the measles agents that reacts specifically in complement fixation tests with convalescent phase measles sera.