THE EFFECT OF IODOACETATE ON THE APPARENT SYNTHESIS OF DIPHOSPHOTHIAMINE DURING THE ESTIMATION OF THE COCARBOXYLASE ACTIVITY OF BLOOD EXTRACT
- 1 October 1949
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Research
- Vol. 27e (5) , 275-280
- https://doi.org/10.1139/cjr49e-037
Abstract
The usual method of determining diphosphothiamine (DPT) by its cocarboxylase activity proved unreliable when applied to blood extract because of a marked upward drift of the results during the period of measurement. This phenomenon, which was associated with excessively high recovery values, was tentatively attributed to a synthesis of DPT since the presence of 1 × 10−3 molar iodoacetate abolished the increase in carboxylase activity. Judging from recovery experiments, the addition of iodoacetate to the reactants permitted a more reliable estimation of the DPT content of blood extract.This publication has 3 references indexed in Scilit:
- The estimation of cocarboxylase (vitamin B1 diphosphate ester) in bloodBiochemical Journal, 1939
- Thyroid and vitamin B1Biochemical Journal, 1939
- Vitamin B1 and cocarboxylase in animal tissuesBiochemical Journal, 1938