CD147/EMMPRIN Acts as a Functional Entry Receptor for Measles Virus on Epithelial Cells
Open Access
- 1 May 2010
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 84 (9) , 4183-4193
- https://doi.org/10.1128/jvi.02168-09
Abstract
Measles is a highly contagious human disease caused by measles virus (MeV) and remains the leading cause of death in children, particularly in developing countries. Wild-type MeV preferentially infects lymphocytes by using signaling lymphocytic activation molecule (SLAM), whose expression is restricted to hematopoietic cells, as a receptor. MeV also infects other epithelial and neuronal cells that do not express SLAM and causes pneumonia and diarrhea and, sometimes, serious symptoms such as measles encephalitis and subacute sclerosing panencephalitis. The discrepancy between the tissue tropism of MeV and the distribution of SLAM-positive cells suggests that there are unknown receptors other than SLAM for MeV. Here we identified CD147/EMMPRIN (extracellular matrix metalloproteinase inducer), a transmembrane glycoprotein, which acts as a receptor for MeV on epithelial cells. Furthermore, we found the incorporation of cyclophilin B (CypB), a cellular ligand for CD147, in MeV virions, and showed that inhibition of CypB incorporation significantly attenuated SLAM-independent infection on epithelial cells, while it had no effect on SLAM-dependent infection. To date, MeV infection was considered to be triggered by binding of its hemagglutinin (H) protein and cellular receptors. Our present study, however, indicates that MeV infection also occurs via CD147 and virion-associated CypB, independently of MeV H. Since CD147 is expressed in a variety of cells, including epithelial and neuronal cells, this molecule possibly functions as an entry receptor for MeV in SLAM-negative cells. This is the first report among members of the Mononegavirales that CD147 is used as a virus entry receptor via incorporated CypB in the virions.Keywords
This publication has 43 references indexed in Scilit:
- Heparin-like glycosaminoglycans prevent the infection of measles virus in SLAM-negative cell linesAntiviral Research, 2008
- Measles virus induces cell-type specific changes in gene expressionVirology, 2008
- Measles Virus N Protein Inhibits Host Translation by Binding to eIF3-p40Journal of Virology, 2007
- A novel monolayer cell line derived from human umbilical cord blood cells shows high sensitivity to measles virusJournal of General Virology, 2007
- Syndecan-1/CD147 association is essential for cyclophilin B-induced activation of p44/42 mitogen-activated protein kinases and promotion of cell adhesion and chemotaxisGlycobiology, 2007
- Redistribution of Cyclophilin A to Viral Factories during Vaccinia Virus Infection and Its Incorporation into Mature ParticlesJournal of Virology, 2003
- Recognition of the Measles Virus Nucleocapsid as a Mechanism of IRF-3 ActivationJournal of Virology, 2002
- CD147 Is a Signaling Receptor for Cyclophilin BBiochemical and Biophysical Research Communications, 2001
- Measles Virus Nucleocapsid Protein Binds to FcγRII and Inhibits Human B Cell Antibody ProductionThe Journal of Experimental Medicine, 1997
- Heterogeneity of Virus Particles in Measles VirusJournal of General Virology, 1979