Sandwich Enzymoimmunoassay of Hepatitis B Surface Antigen (HBsAg)
- 1 October 1978
- journal article
- research article
- Published by Wiley in Vox Sanguinis
- Vol. 35 (4) , 219-223
- https://doi.org/10.1111/j.1423-0410.1978.tb02925.x
Abstract
A sandwich enzymoimmunoassay (EIA) procedure was developed for the detection of HBsAg using Fab'' of rabbit anti-HBsAg conjugated with .beta.-D-galactosidase from Escherichia coli with anti-HBsA-coated silicone rubber discs as a solid phase. EIA could detect 1 ng/ml of HBsAg. It was as sensitive as radioimmunoassay (RIA, AusRia II) and about 30-fold more sensitive than reversed passive hemagglutination assay (RPHA, ReverseCell). EIA and RIA could detect more HBsAg-positive sera than RPHA.This publication has 16 references indexed in Scilit:
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