Acidic and Basic Extracellular Pathogenesis-Related Leaf Proteins from Fifteen Potato Cultivars

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Abstract
Fifteen potato [Solanum tuberosum] cultivars (Avon, Bintje, Cherokee, Green Mountain, Hudson, Jemseg, Kennebec, Nooksack, Norchip, Norland, Sable, Saco, Sebago, Superior, and Yukon Gold) produced necrotic local lesions on leaves inoculated with high concentrations (20 mg/ml) of U2 tobacco mosaic virus. Proteins in the intercellular fluid extracts from inoculated leaves were characterized by native and denaturing polyacrylamide gel electrophoresis, gel filtration, and chromatofocusing. The presence of glycoproteins was also tested. Nine acidic (apparent isoelectric points [pI] 3.95-5.60), a low molecular weight (28,200-40,700) soluble proteins, and six major basic proteins (apparent pI 7.2-9.2, Mr 14,100-33,300) were found in addition to several minor ones. Four acidic proteins with Rf 0.54 (Mr 38,900, apparent pI 3.95), 0.56 (Mr 40,700, apparent pI 4.10), 0.59 (Mr 40,700, apparent pI 4.0), and 0.62 (Mr 40,700, apparent pI 3.95) could be used as genetic markers to identify cultivars. No acidic proteins were observed that were equivalent to the b1, b2 and b3 (pathogenesis-related 1a,b,c) proteins found in various Nicotiana species. Three acidic peroxidases (glycoproteins) (Rf 0.09, 0.39. and 0.42) were identified.

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