Determination and comparative analysis of the catalytic subunit of adenosine 3′,5′-cyclic phosphate-dependent protein kinase by an enzyme-linked immunosorbent assay
- 15 October 1982
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 208 (1) , 109-117
- https://doi.org/10.1042/bj2080109
Abstract
A specific antiserum against bovine heart catalytic subunit was used for the determination of the catalytic subunit in an enzyme-linked immunosorbent assay. Under the conditions elaborated the assay has a lower detection limit for catalytic subunit of 0.25 pmol/ml. In crude bovine heart extracts the concentration of catalytic subunit was determined by this method to be 0.18 +/- 0.02 mumol/kg wet wt. The immunochemical comparison of various animal species and cells, including organisms like amoebae and yeast, shows the broad applicability of the assay and provides evidence that the catalytic subunit is a highly conserved molecule.This publication has 34 references indexed in Scilit:
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